5-Methyltetrahydrofolic Acid

About 5-Methyltetrahydrofolic Acid

5-Methyltetrahydrofolic Acid is an impurity of Folic Acid carrying the CAS registry number 134-35-0. The compound has the formula C20H25N7O6, corresponding to a molecular weight of 459.5 g/mol. Common synonyms include N-Methyltetrahydrofolic Acid and Prefolic A.

The product is provided as a solid. Keep the vial tightly closed, protected from light and humidity, at the temperature stated on the Certificate of Analysis. It carries the GHS signal word “Warning” with 1 hazard statement. Review the Safety Data Sheet before use.

Pharmaceutical QC and development laboratories use this impurity standard of Folic Acid to develop and validate HPLC methods, to set specification limits and to document impurity profiles in ANDA and DMF submissions. Our customers for this product work in pharmaceutical manufacturing and academic and industrial research.

Available in 10 mg, 25 mg, 50 mg and 100 mg packs, 5-Methyltetrahydrofolic Acid is dispatched with a Certificate of Analysis (COA) to destinations worldwide.

Typical applications

Analytical method development
Method validation (AMV)
QC release testing
ANDA / DMF filings

Industries

Related to Folic Acid

View every impurity, metabolite and intermediate we list for Folic Acid.

Folic Acid impurities

Specifications & identifiers

Chemical identifiers
Product name	5-Methyltetrahydrofolic Acid
Catalogue No.	VQ-030859
CAS Number	134-35-0
IUPAC name	(2S)-2-[[4-[(2-amino-5-methyl-4-oxo-3,6,7,8-tetrahydropteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid
Molecular formula	C₂₀H₂₅N₇O₆
Molecular weight	459.5 g/mol
Exact mass	459.1866
Grade	Reference Standard
HSN code	38229090
InChIKey	ZNOVTXRBGFNYRX-ABLWVSNPSA-N
SMILES	CN1C(CNC2=C1C(=O)NC(=N2)N)CNC3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
PubChem CID	135415868

Physical properties
Physical form	Solid
LogP (XLogP3)	-0.5
H-bond donors / acceptors	7 / 10
TPSA	198 Å²
Synonyms	N-Methyltetrahydrofolic Acid, Prefolic A, n5-methyltetrahydrofolate, n5-methyltetrahydrofolic acid, n-methyltetrahydrofolate, N5-Methyltetrahydropteroylglutamate, 5-methyltetrahydropteroylglutamic acid, 5-methyl-5,6,7,8-tetrahydropteroyl-L-glutamic acid, 5-methyl-THF, Methyl folate, 5-Methyltetrahydrofolate, 5-Methyl THF

Safety & hazard information (GHS)

Harmful / irritant

Signal word: Warning

Hazard statements

H317May cause an allergic skin reaction

Precautionary statements: P261, P272, P280, P302 + P352, P321, P333 + P317, P362 + P364, P501

Classification is indicative (aggregated GHS notifications) and may vary by grade and concentration. Always refer to the SDS supplied with your lot.

Documents

Safety Data Sheet (SDS)16-section GHS · on request Certificate of AnalysisTypical / batch COA · on request Specification sheetTDS / MOA · on request

Frequently asked questions

What is the CAS number of 5-Methyltetrahydrofolic Acid?

The CAS Registry Number of 5-Methyltetrahydrofolic Acid is 134-35-0. It is also known as N-Methyltetrahydrofolic Acid, Prefolic A, n5-methyltetrahydrofolate.

What is the molecular formula and molecular weight of 5-Methyltetrahydrofolic Acid?

5-Methyltetrahydrofolic Acid has the molecular formula C20H25N7O6 and a molecular weight of 459.5 g/mol. Its IUPAC name is (2S)-2-[[4-[(2-amino-5-methyl-4-oxo-3,6,7,8-tetrahydropteridin-6-yl)methylamino]benzoyl]amino]pentanedioic acid.

What is 5-Methyltetrahydrofolic Acid used for?

5-Methyltetrahydrofolic Acid is supplied as a characterised reference material related to Folic Acid. It is used for analytical method development and validation (AMV), impurity profiling, stability studies and quality control, and in ANDA/DMF regulatory filings.

Which grade and pack sizes of 5-Methyltetrahydrofolic Acid can I order?

We supply 5-Methyltetrahydrofolic Acid in Reference Standard grade, in 10 mg, 25 mg, 50 mg, 100 mg packs. Larger bulk quantities and custom packing are quoted on request.

Do you provide a COA and SDS with 5-Methyltetrahydrofolic Acid?

Yes. Every lot of 5-Methyltetrahydrofolic Acid is shipped with a batch-specific Certificate of Analysis, and a 16-section GHS Safety Data Sheet is provided with the quotation and the shipment.

Can 5-Methyltetrahydrofolic Acid be shipped internationally?

Yes. 5-Methyltetrahydrofolic Acid is classified as hazardous, so it is packed in UN-approved packaging and shipped as dangerous goods under IATA/IMDG rules. We export worldwide on EXW, FOB, CIF and DAP terms.