Nitrosobiotin

About Nitrosobiotin

Nitrosobiotin is a nitrosamine impurity of Biotin carrying the CAS registry number 56859-26-8. With the molecular formula C10H15N3O4S, it has a molar mass of 273.31 g/mol. Its systematic (IUPAC) name is 5-[(3aR,6S,6aS)-3-nitroso-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]pentanoic acid. Common synonyms include 1'-Nitroso Biotin and 1'-Nitrosobiotin.

The product is provided as a solid. Store the standard in its sealed vial, protected from light and moisture, and follow the storage condition printed on the COA. The GHS classification uses the signal word “Warning”, so appropriate gloves, eye protection and ventilation are recommended.

It is used as a reference standard for trace-level detection and quantitation of nitrosamine impurities by LC-MS/MS or GC-MS, supporting nitrosamine risk assessments for Biotin, method validation (AMV) and regulatory filings. Typical end-use sectors are pharmaceutical manufacturing and academic and industrial research.

Standard pack sizes are 10 mg, 25 mg, 50 mg and 100 mg, and every batch ships with a Certificate of Analysis (COA) and we export worldwide.

Typical applications

Nitrosamine risk assessment
Trace LC-MS/MS quantitation
Method validation (AMV)
Regulatory submissions

Industries

Related to Biotin

View every impurity, metabolite and intermediate we list for Biotin.

Biotin impurities

Specifications & identifiers

Chemical identifiers
Product name	Nitrosobiotin
Catalogue No.	VQ-024600
CAS Number	56859-26-8
IUPAC name	5-[(3aR,6S,6aS)-3-nitroso-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]pentanoic acid
Molecular formula	C₁₀H₁₅N₃O₄S
Molecular weight	273.31 g/mol
Exact mass	273.0783
Grade	Reference Standard
HSN code	38229090
InChIKey	IFUXQNNMTPBRPW-ZKWXMUAHSA-N
SMILES	C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)O)NC(=O)N2N=O
PubChem CID	191424

Physical properties
Physical form	Solid
LogP (XLogP3)	0.2
H-bond donors / acceptors	2 / 6
TPSA	124 Å²

Safety & hazard information (GHS)

Harmful / irritant

Health hazard

Signal word: Warning

Hazard statements

H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
H351Suspected of causing cancer

Precautionary statements: P203, P261, P264, P264 + P265, P271, P280, P302 + P352, P304 + P340, P305 + P351 + P338, P318, P319, P321, P332 + P317, P337 + P317, P362 + P364, P403 + P233, P405, P501

Classification is indicative (aggregated GHS notifications) and may vary by grade and concentration. Always refer to the SDS supplied with your lot.

Documents

Safety Data Sheet (SDS)16-section GHS · on request Certificate of AnalysisTypical / batch COA · on request Specification sheetTDS / MOA · on request

Frequently asked questions

What is the CAS number of Nitrosobiotin?

The CAS Registry Number of Nitrosobiotin is 56859-26-8. It is also known as 1'-Nitroso Biotin, 1'-Nitrosobiotin, 5-[(3as,4s,6ar)-1-nitroso-2-oxohexahydro-1h-thieno[3,4-d]imidazol-4-yl]pentanoic acid.

What is the molecular formula and molecular weight of Nitrosobiotin?

Nitrosobiotin has the molecular formula C10H15N3O4S and a molecular weight of 273.31 g/mol. Its IUPAC name is 5-[(3aR,6S,6aS)-3-nitroso-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]pentanoic acid.

What is Nitrosobiotin used for?

Nitrosobiotin is supplied as a characterised reference material related to Biotin. It is used for analytical method development and validation (AMV), impurity profiling, stability studies and quality control, and in ANDA/DMF regulatory filings.

Which grade and pack sizes of Nitrosobiotin can I order?

We supply Nitrosobiotin in Reference Standard grade, in 10 mg, 25 mg, 50 mg, 100 mg packs. Larger bulk quantities and custom packing are quoted on request.

Do you provide a COA and SDS with Nitrosobiotin?

Yes. Every lot of Nitrosobiotin is shipped with a batch-specific Certificate of Analysis, and a 16-section GHS Safety Data Sheet is provided with the quotation and the shipment.

Can Nitrosobiotin be shipped internationally?

Yes. Nitrosobiotin is classified as hazardous, so it is packed in UN-approved packaging and shipped as dangerous goods under IATA/IMDG rules. We export worldwide on EXW, FOB, CIF and DAP terms.