Methotrexate α-Ethyl Ester

Name: Methotrexate α-Ethyl Ester
Brand: Vaqora Chemicals
SKU: VQ-031828

About Methotrexate α-Ethyl Ester

Methotrexate α-Ethyl Ester is an impurity of Methotrexate carrying the CAS registry number 65148-63-2. Formula C22H26N8O5, molecular weight 482.5 g/mol. The IUPAC name is (4S)-4-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]-5-ethoxy-5-oxopentanoic acid. Alternative names: alpha-Monoethyl methotrexate and Methotrexate alpha-monoethyl ester.

It is supplied as a solid. Reference materials should be stored sealed and away from light and moisture, as specified on the accompanying COA. Good laboratory practice and suitable PPE are recommended during handling.

This Methotrexate impurity supports analytical method development and validation (AMV), batch release testing and the impurity sections of ANDA and DMF filings. It is used across pharmaceutical manufacturing and academic and industrial research.

Standard pack sizes are 10 mg, 25 mg, 50 mg and 100 mg, and every batch ships with a Certificate of Analysis (COA) and we export worldwide.

Typical applications

Analytical method development
Method validation (AMV)
QC release testing
ANDA / DMF filings

Industries

Related to Methotrexate

View every impurity, metabolite and intermediate we list for Methotrexate.

Methotrexate impurities

Specifications & identifiers

Chemical identifiers
Product name	Methotrexate α-Ethyl Ester
Catalogue No.	VQ-031828
CAS Number	65148-63-2
IUPAC name	(4S)-4-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]-5-ethoxy-5-oxopentanoic acid
Molecular formula	C₂₂H₂₆N₈O₅
Molecular weight	482.5 g/mol
Exact mass	482.2026
Grade	Reference Standard
HSN code	38229090
InChIKey	MVIRCEDDERYMPC-HNNXBMFYSA-N
SMILES	CCOC(=O)[C@H](CCC(=O)O)NC(=O)C1=CC=C(C=C1)N(C)CC2=CN=C3C(=N2)C(=NC(=N3)N)N
PubChem CID	90672325

Physical properties
Physical form	Solid
LogP (XLogP3)	0.3
H-bond donors / acceptors	4 / 12
TPSA	200 Å²

Documents

Safety Data Sheet (SDS)16-section GHS · on request Certificate of AnalysisTypical / batch COA · on request Specification sheetTDS / MOA · on request

Frequently asked questions

What is the CAS number of Methotrexate α-Ethyl Ester?

The CAS Registry Number of Methotrexate α-Ethyl Ester is 65148-63-2. It is also known as alpha-Monoethyl methotrexate, Methotrexate alpha-monoethyl ester, 1-Ethyl N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamate.

What is the molecular formula and molecular weight of Methotrexate α-Ethyl Ester?

Methotrexate α-Ethyl Ester has the molecular formula C22H26N8O5 and a molecular weight of 482.5 g/mol. Its IUPAC name is (4S)-4-[[4-[(2,4-diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]-5-ethoxy-5-oxopentanoic acid.

What is Methotrexate α-Ethyl Ester used for?

Methotrexate α-Ethyl Ester is supplied as a characterised reference material related to Methotrexate. It is used for analytical method development and validation (AMV), impurity profiling, stability studies and quality control, and in ANDA/DMF regulatory filings.

Which grade and pack sizes of Methotrexate α-Ethyl Ester can I order?

We supply Methotrexate α-Ethyl Ester in Reference Standard grade, in 10 mg, 25 mg, 50 mg, 100 mg packs. Larger bulk quantities and custom packing are quoted on request.

Do you provide a COA and SDS with Methotrexate α-Ethyl Ester?

Yes. Every lot of Methotrexate α-Ethyl Ester is shipped with a batch-specific Certificate of Analysis, and a 16-section GHS Safety Data Sheet is provided with the quotation and the shipment.

Can Methotrexate α-Ethyl Ester be shipped internationally?

Yes. We export Methotrexate α-Ethyl Ester worldwide by air and sea freight or express courier, on EXW, FOB, CIF, CFR or DAP Incoterms, with full export documentation.