7-Hydroxy Methotrexate

About 7-Hydroxy Methotrexate

7-Hydroxy Methotrexate is a metabolite or degradation product of Methotrexate carrying the CAS registry number 5939-37-7. Its molecular formula is C20H22N8O6 and its molecular weight is 470.4 g/mol. Its systematic (IUPAC) name is (2S)-2-[[4-[(2,4-diamino-7-oxo-8H-pteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid. It is also known as Methotrexate 7-Hydroxy Metabolite or Methotrexate 7-Hydroxy.

It is supplied as a solid. Keep the vial tightly closed, protected from light and humidity, at the temperature stated on the Certificate of Analysis. Handle it with standard laboratory precautions and appropriate personal protective equipment.

Analytical teams use this compound to identify metabolites and degradants of Methotrexate, to validate stability-indicating methods (AMV) and to support QC and regulatory filings. It is used across pharmaceutical manufacturing and academic and industrial research.

Standard pack sizes are 10 mg, 25 mg, 50 mg and 100 mg, and every batch ships with a Certificate of Analysis (COA) and we export worldwide.

Typical applications

Metabolite identification
Stability-indicating methods
Impurity profiling

Industries

Related to Methotrexate

View every impurity, metabolite and intermediate we list for Methotrexate.

Methotrexate impurities

Specifications & identifiers

Chemical identifiers
Product name	7-Hydroxy Methotrexate
Catalogue No.	VQ-031825
CAS Number	5939-37-7
IUPAC name	(2S)-2-[[4-[(2,4-diamino-7-oxo-8H-pteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid
Molecular formula	C₂₀H₂₂N₈O₆
Molecular weight	470.4 g/mol
Exact mass	470.1662
Grade	Reference Standard
HSN code	38229090
InChIKey	HODZDDDNGRLGSI-NSHDSACASA-N
SMILES	CN(CC1=NC2=C(N=C(N=C2NC1=O)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
PubChem CID	5484402

Physical properties
Physical form	Solid
LogP (XLogP3)	-0.8
H-bond donors / acceptors	6 / 12
TPSA	226 Å²
Synonyms	Methotrexate 7-Hydroxy Metabolite, Methotrexate 7-Hydroxy, 7-OH-methotrexate, 7-Hydroxymethotrexat, Glutamic acid, N-(p-(((2,4-diamino-7-hydroxy-6-pteridinyl)methyl)methylamino)benzoyl)-, DISCONTINUED, Please see H945926, (4-(((2,4-diamino-7-hydroxypteridin-6-yl)methyl)(methyl)amino)benzoyl)-L-glutamic acid, N-(4-{[(2,4-Diamino-7-hydroxypteridin-6-yl)methyl](methyl)amino}benzoyl)-L-glutamic acid

Documents

Safety Data Sheet (SDS)16-section GHS · on request Certificate of AnalysisTypical / batch COA · on request Specification sheetTDS / MOA · on request

Frequently asked questions

What is the CAS number of 7-Hydroxy Methotrexate?

The CAS Registry Number of 7-Hydroxy Methotrexate is 5939-37-7. It is also known as Methotrexate 7-Hydroxy Metabolite, Methotrexate 7-Hydroxy, 7-OH-methotrexate.

What is the molecular formula and molecular weight of 7-Hydroxy Methotrexate?

7-Hydroxy Methotrexate has the molecular formula C20H22N8O6 and a molecular weight of 470.4 g/mol. Its IUPAC name is (2S)-2-[[4-[(2,4-diamino-7-oxo-8H-pteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic acid.

What is 7-Hydroxy Methotrexate used for?

7-Hydroxy Methotrexate is supplied as a characterised reference material related to Methotrexate. It is used for analytical method development and validation (AMV), impurity profiling, stability studies and quality control, and in ANDA/DMF regulatory filings.

Which grade and pack sizes of 7-Hydroxy Methotrexate can I order?

We supply 7-Hydroxy Methotrexate in Reference Standard grade, in 10 mg, 25 mg, 50 mg, 100 mg packs. Larger bulk quantities and custom packing are quoted on request.

Do you provide a COA and SDS with 7-Hydroxy Methotrexate?

Yes. Every lot of 7-Hydroxy Methotrexate is shipped with a batch-specific Certificate of Analysis, and a 16-section GHS Safety Data Sheet is provided with the quotation and the shipment.

Can 7-Hydroxy Methotrexate be shipped internationally?

Yes. We export 7-Hydroxy Methotrexate worldwide by air and sea freight or express courier, on EXW, FOB, CIF, CFR or DAP Incoterms, with full export documentation.